Vol. 7 (3) : May-June 2016 issue
Green Farming Vol. 7 (3) : 738-742 ; May-June, 2016
Supercritical fluid extraction (SFE) of bioactive flavonoids from mulberry (Morus alba L.) leaf powder
RAMYA V.a1, UDAYKUMAR NIDONIa2, JAYARAMA NAIK N.b3, ASHOKA J.c4, SHARANAGOUDA HIREGOUDARa5, RAMACHANDRA C.T.a6, LAVANYA V.a7 and GOUDRA PROMOD GOUDAa8
aDeptt. of Processing and Food Engineering., cDeptt. of Entomology, College of Agricultural Engineering, University of Agricultural Sciences, Raichur - 584 104 (Karnataka)
bDepartment of Food Technology, Davangere University, Davangere - 577 002 (Karnataka)
Designation : 1,8Ph.D. Scholar *(rajramya75@gmail.com), CTAE, MPUAT, Udaipur 2,4Head, 3Res. Scholar, 5,6Asstt. Professor, 7M-Tech Student (CTAE, MPUAT, Udaipur)
Subject : Food Science, Preservation & Processing, Protectants & Infestation and Post-harvest Technology
Paper No. : P-3991
Total Pages : 5
Received : 21 July 2015
Revised accepted : 13 April 2016
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Citation :
RAMYA V., UDAYKUMAR NIDONI, JAYARAMA NAIK N., ASHOKA J., SHARANAGOUDA HIREGOUDAR, RAMACHANDRA C.T., LAVANYA V. and GOUDRA PROMOD GOUDA. 2016. Supercritical fluid extraction (SFE) of bioactive flavonoids from mulberry (Morus alba L.) leaf powder. Green Farming Vol. 7 (3) : 738-742 ; May-June, 2016
ABSTRACT
Supercritical fluid extraction (SFE) by using CO2 as a solvent, a relatively new separation technique was used in the present study to obtain bioactive flavonoid compounds from dry leaves of Morus alba. Since carbon dioxide is a non-polar solvent, ethanol was used as co-solvent to increase the polarity of the fluid. The studied parameters were pressure (100, 150 and 200 bar), temperature (40, 50 and 60 °C) and dynamic extraction time (40, 60 and 80 min). The optimum extraction condition occurred at 200 bar, 50 °C and 80 min. Extracted yield at optimum condition was then analyzed by high performance liquid chromatography (HPLC) and spectrophotometer for quantifying bioactive flavonoid compounds. The results indicated that SC-CO2 extraction is a promising and alternative process for recovering the bioactive compounds from mulberry leaves.
Key words :
Bioactive flavonoid, Carbon dioxide, High performance liquid chromatography, Supercritical fluid extraction.
