Vol. 5 (6) : November-December 2014 issue
Green Farming Vol. 5 (6) : 1092-1095 ; November-December, 2014
Survivability of arbuscular mycorrhiza fungi Glomus intraradices mass cultured by different culturing methods
R.P. AHIRWAR1*, N.G. MITRA2, A. SHARMA3 and A.R. WASNIKER4
Department of Soil Science and Agricultural Chemistry, Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur - 382 004 (Madhya Pradesh)
Designation : 1Tech. Officer *(rameshwar_jan@rediffmail.com), 2Professor, 3Res. Scholar, 4Asstt. Professor
Subject : Soil Science & Agric. Chemistry; Soil Health & Soil Conservation Engg.
Paper No. : P-1826
Total Pages : 4
Received : 14 April 2014
Revised accepted : 28 October 2014
Get Access
Citation :
R.P. AHIRWAR, N.G. MITRA, A. SHARMA and A.R. WASNIKER. 2014. Survivability of arbuscular mycorrhiza fungi Glomus intraradices mass cultured by different culturing methods. Green Farming Vol. 5 (6) : 1092-1095 ; November-December, 2014
ABSTRACT
The present investigation was carried out in a pot experiment following simple randomized block design with three replications. In all, sixteen treatment combinations with one dummy control were incorporated with two types of biotic inoculants (pot culture AMF1 and root-organ culture AMF2 of mycorrhiza), and two types abiotic nutrient supplements of phosphorus and zinc each at four levels (0, 30, 60, 90 kg P2O5 ha-1 and 0, 25, 50, 75 kg Zn ha-1). Prior to bio-inoculation to soil, the mycorrhiza was mass-produced in vivo in carrot roots and in vitro in axenic culture media. The count of mycorrhizal propagules was increased by 83.27% with pot culture mycorrhizal inoculation supplemented with 60 kg ha-1 P2O5 (AMF1 + P2), when root culture mycorrhiza with 30 kg P2O5 ha-1 (AMF1 + P1) increased propagule counts by 58.32% over that from the lowest performing treatment AMF2 + P0 (126.2 propagules/100 g soil).
Key words :
AMF, Chlamydospore, Mycorrhiza, Propagules, Pot culture, Root-organ culture.
