The slurry released after methane production contains potent Methanobacterium which play an important role in achieving India's increased levels of ambition in the waste management area and in achieving the goal of more yield of vegetation and reduced pollution. This proposal studies on how biogas waste slurry can be utilized as an efficient carrier for nano-encapsulated bio-control agents. We found that a system of collecting organic waste into pilot scale biogas plants and applying the left over slurry directly to plants as fertilizers is a common practice. The potency of slurry as suitable carrier for bio control agents by in vitro treatments is not investigated. These slurry carriers after fermentation produce a liquid organic fertilizer with increased shelf-life. A cost effective carrier with an essential mineral composition is required for them which on fermentation act as fertilizer on par with pesticides. Introducing this formula to agriculture markets require sufficient experiments to prove their efficacy to available organic liquid fertilizers like Bacillus subtilis composting tonic. The bio-control agents when treated with coir pith are protected from external biome and show targeted release of nutrients to plants there by retaining its moisture and shelf-life. In this study we use Cellulosimicrobium sp. and Pseudomonas sp. as biocontrol sample.
At regular intervals they are nano-encapsulated with chitosan polymers in vitro and fermented in biogas slurry for 7 days. These inoculums in the ratio of 3 ml per 1000 ml of slurry will be prepared on a large scale. Moreover they will be packed in bottles and supplied to farmers. Further they may be introduced in to fields as seed treatments and soil application. The biometric observations like length of the plant, number of leaves and fruit weight attributes are to be studied and plotted with MSTAT. Confirmatory studies are to be done on replications of Ananas comosus fruit crop. Readings were taken for a period of 60 days on parameters like length of the plant and number of leaves of this tissue culture variety of pineapple. A comparative study was done with effective microorganisms (EM)-Hariyali treatments and biogas slurry treatments. EM solution treatments showed enhanced growth compared to slurry. The control plants were maintained without any biotic or abiotic stresses.