Vol. 1 (6) : November-December 2010 issue
Green Farming Vol. 1 (6) : 551-554 (November-December, 2010) (New Series)
Transformation of mothbean (Vigna aconitifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens
S. KUMARa1, G. SINGHa2*, R.S. JATb3, S.N. SAXENAa4 and R. SHARMAa5
aDepartment of Biotechnology, College of Agriculture, Rajasthan Agricultural University, Bikaner – 334 006 (Rajasthan)
bNRC Plant Biotechnology, Indian Agriculture Research Institute, New Delhi - 110 012
Designation : 1Ph.D. Scholar, 2Professor, 3,4Sr. Scientist, 5 Associate Professor
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. :
Total Pages : 4
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Revised accepted :
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Citation :
S. KUMAR, G. SINGH, R.S. JAT, S.N. SAXENA and R. SHARMA. 2010. Transformation of mothbean (Vigna aconitifolia) cotyledonary nodes through vacuum infiltration of Agrobacterium tumefaciens. Green Farming Vol. 1 (6) : 551-554 ; November-December, 2010 (New Series)
ABSTRACT
The present investigation generates a rapid and convenient stable expression protocol based on vacuum infiltration of Agrobacterium tumefaciens cells into moth bean cotyledonary nodes. Vacuum infiltrated tissues showed transient GUS expression than did non infiltrated tissues. The leaflets arising from the transformed shoots on kanamycin supplemented media were found GUS positive during histochemical assay. We believe this to be the first report of this technique for putative gene expression in mothbean cotyledonary nodes. Mothbean cotyledonary nodes are one of the most regenerative tissues in legumes.
Key words :
Agrobacterium tumefaciens, Cotyledonary nodes, Mothbean, Vacuum infiltration, Vigna aconitifolia.
