Vol. 7 (2) : March-April 2016 issue
Green Farming Vol. 7 (2) : 379-382 ; March-April, 2016
Validation of SCAR markers linked to anthracnose resistant in French bean (Phaseolus vulgaris L.) germplasm
MANJULA V.1*, SHYAMALAMMA S.2, AKSHATA HOSAMANI3 and GEETHA M.S.4
Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru - 560 065 (Karnataka).
Designation : 1,3,4P.G. Student *(meetmanjuv@gmail.com), 2Assoc. Professor & Principal Scientist
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-3841
Total Pages : 4
Received : 23 June 2015
Revised accepted : 25 February 2016
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Citation :
MANJULA V., SHYAMALAMMA S., AKSHATA HOSAMANI and GEETHA M.S. 2016. Validation of SCAR markers linked to anthracnose resistant in French bean (Phaseolus vulgaris L.) germplasm. Green Farming Vol. 7 (2) : 379-382 ; March-April, 2016
ABSTRACT
French bean is one of the major leguminous crop grown for its high nutritive value, short duration and high production potential. The bean production is affected by a destructive seed borne fungal disease anthracnose caused by Colletotrichum lindemuthianum (Sacc. and Magn.). Therefore to evolve bean varieties resistant to anthracnose and to identify the source of disease resistance using SCAR markers associated with anthracnose resistant genes trails was conducted. Forty French bean accessions were screened along with two resistance genotypes viz., D line, L line, two susceptible varieties viz., Kanchana, Jwala were used as checks. Plants were inoculated with isolated Colletotrichum lindemuthianum spore culture and disease scoring was done. Fifteen SCAR markers were used for screening forty French bean accessions, out of which six markers viz., SAS13, SBB14, SC08, SF10, SW12 and SZ04 showed good amplification between resistant and susceptible genotypes. The product size varied from 567 bp to 1150 bp with different genes viz., Co-42, Co-4, Co-10, Co-3/Co-9 and Co-6 linked to anthracnose resistance.
Key words :
Anthrancose resistant, Colletotrichum lindemuthianum, French bean, SCAR markers, Validation.