The present investigation was carried out in vitro lab experiment following simple Randomized Block Design with three replications. In all, sixteen treatment combinations with one dummy control were incorporated. To test longevity of Azotobacter for two types of cultures (Liquid and carrier based cultures of Azotobacter), and two types inducers physical and chemical inducers were used. The physical inducer used was temperature for storage of Azotobacter in liquid and carrier based culture at 40C (in refrigerator) – T0, at 20, 30, 40 oC (in incubator) – T1, T2, T3 .The chemical inducers used were n-butanol (0.3%), ?-hydroxy butyrate (each 0.3%), and mineral Ca deficiency in culture media – C1, C2, C3, and C0 (for no chemical inducer i.e., normal medium).At one month interval of microbial growth in carrier based and liquid cultures of Azotobacter the viable cell count of the bacteria was in the range 7.19 log cfu/g (1.55x107 cfu/g) to 9.90 log cfu/ml (7.94x109cfu/ml). The minimum was with treatment combination of carrier-based bioinoculant supplemented with n-butanol and the maximum with liquid based culture maintained at 30oC, respectively. Thereafter, since second month interval the microbial growth decreased continuously till the last observation at six moth growth interval. At the last observation the microbial population reached in a range of 3.18 log cfu/g (1.51x103 cfu/gm) to 5.86 log cfu/ml (7.24x105cfu/ml) with the treatment combinations carrier-based bioinoculants with n-butanol and liquid based culture at 30oC, respectively.Mean values of viable cell count (average of one to six month intervals) were observed in a range of 4.99 log cfu/g (9.71x104 cfu/g) to 7.67 log cfu/ml (4.70x107cfu/ml) with different treatments. The maximum was observed with liquid at 30oC which was significantly higher as compared to other treatments and the minimum value was recorded with carrier based bioinoculant with n-butanol treatment.